Jurin Laksanawisit, chief opposition whip, yesterday submitted the petition, signed by 146 Democrat MPs, to Deputy Senate Speaker Surachai Liengboonlertchai in an attempt to remove Ms Yingluck, Deputy Prime Minister Plodprasop Suraswadi and Interior Minister Charupong Ruangsuwan.
Mr Jurin refused to disclose details of the petition but merely said it was confidential.
Mr Surachai said he would check the authenticity of the petition before forwarding it to the National Anti-Corruption Commission within 15 days.
If the three Cabinet members are found guilty, the Senate will be obliged to dismiss them, he said.
The Democrat Party later submitted a request for a non-confidence debate against the prime minister and interior minister.
The proposal was handed over to House Speaker Somsak Kiatsuranont who would not commit on when the censure debate will be held.
Somsak said he would convene a meeting of legal advisors on Monday to check the validity of the motion.
Mr Jurin said opposition MPs should be allowed to fully debate against the prime minister who should make herself present at the House of Representatives to personally give her clarifications.